Chemicals Formula

Thursday, March 12, 2009

5-Minute Reversible™ Zinc Stain Kit

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A single step stain for the rapid detection of proteins separated by SDS-denatured or native PAGE. Staining is based on the interaction of zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white. The charge coating the protein prevents the stain from binding to the protein. Clear protein bands are visualized within 10 - 15 minutes. Staining does not interfere with electroelution of proteins or alter their biological properties. Destaining, if needed, can be accomplished within 5 minutes.

Sensitivity: 8 - 12 ng protein.
Kit contents: 5-Minute Reversible™ Zinc Stain, zinc reagent I and II, destaining solution, and a directional insert.
Note: one kit provides reagents sufficient to stain 25 mini gels.

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