Chemicals Formula

Thursday, March 12, 2009

5-Minute Reversible™ Copper Stain Kit

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A single step stain for the rapid detection of proteins separated by SDS-PAGE. The stain is better suited for gels =12%. Staining is based on the interaction of copper ions with polyacrylamide and proteins. The stain works by depositing a copper metal precipitate in the gel, which turns the gel opaque blue-green. The SDS coating on the proteins prevents the stain from binding to the protein. Clear protein bands are visualized within 5 minutes. Staining does not interfere with electroelution of proteins or alter their biological properties. Destaining, if needed, can be accomplished within 20 - 25 minutes.
Sensitivity: 8 - 12 ng protein .
Kit contents: 5-Minute Reversible™ Copper Stain, destaining solution, and a directional insert.

Note: One kit provides reagents sufficient to stain 25 mini gels.
Risk and Safety Statements: R: 22-36/37/38; S: 26-3

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