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A single step stain for the rapid detection of proteins separated by SDS-denatured or native PAGE. Staining is based on the interaction of zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white. The charge coating the protein prevents the stain from binding to the protein. Clear protein bands are visualized within 10 - 15 minutes. Staining does not interfere with electroelution of proteins or alter their biological properties. Destaining, if needed, can be accomplished within 5 minutes.
Sensitivity: 8 - 12 ng protein.
Kit contents: 5-Minute Reversible™ Zinc Stain, zinc reagent I and II, destaining solution, and a directional insert.
Note: one kit provides reagents sufficient to stain 25 mini gels.
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A single step stain for the rapid detection of proteins separated by SDS-PAGE. The stain is better suited for gels =12%. Staining is based on the interaction of copper ions with polyacrylamide and proteins. The stain works by depositing a copper metal precipitate in the gel, which turns the gel opaque blue-green. The SDS coating on the proteins prevents the stain from binding to the protein. Clear protein bands are visualized within 5 minutes. Staining does not interfere with electroelution of proteins or alter their biological properties. Destaining, if needed, can be accomplished within 20 - 25 minutes.
Sensitivity: 8 - 12 ng protein .
Kit contents: 5-Minute Reversible™ Copper Stain, destaining solution, and a directional insert.
Note: One kit provides reagents sufficient to stain 25 mini gels.
Risk and Safety Statements: R: 22-36/37/38; S: 26-3
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